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  1. Content type: Methodology

    As engineered biological systems become more complex, it is increasingly common to express multiple operons from different plasmids and inducible expression systems within a single host cell. Optimizing such s...

    Authors: Taek Soon Lee, Rachel A Krupa, Fuzhong Zhang, Meghdad Hajimorad, William J Holtz, Nilu Prasad, Sung Kuk Lee and Jay D Keasling

    Citation: Journal of Biological Engineering 2011 5:12

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  2. Content type: Research

    Stink bugs (Hemiptera: Pentatomidae) comprise a critically important insect pest complex affecting 12 major crops worldwide including cotton. In the US, stink bug damage to developing cotton bolls causes boll ...

    Authors: Jinjun Xia, Adnan Mustafic, Michael D Toews and Mark A Haidekker

    Citation: Journal of Biological Engineering 2011 5:11

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  3. Content type: Research

    Engineers frequently vary design parameters to optimize the behaviour of a system. However, synthetic biologists lack the tools to rapidly explore a critical design parameter, gene expression level, and have n...

    Authors: Joshua T Kittleson, Sherine Cheung and JChristopher Anderson

    Citation: Journal of Biological Engineering 2011 5:10

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  4. Content type: Letter to the editors

    Members of the synthetic biology community have discussed the significance of word selection when describing synthetic biology to the general public. In particular, many leaders proposed the word "create" was ...

    Authors: Brianna Pearson, Sam Snell, Kyri Bye-Nagel, Scott Tonidandel, Laurie J Heyer and A Malcolm Campbell

    Citation: Journal of Biological Engineering 2011 5:9

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  5. Content type: Research

    Bacterial cell lysis is a widely studied mechanism that can be achieved through the intracellular expression of phage native lytic proteins. This mechanism can be exploited for programmed cell death and for ge...

    Authors: Lorenzo Pasotti, Susanna Zucca, Manuel Lupotto, Maria Gabriella Cusella De Angelis and Paolo Magni

    Citation: Journal of Biological Engineering 2011 5:8

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  6. Content type: Research

    FeFe-hydrogenases are the most active class of H2-producing enzymes known in nature and may have important applications in clean H2 energy production. Many potential uses are currently complicated by a crucial we...

    Authors: Buz Barstow, Christina M Agapakis, Patrick M Boyle, Gerald Grandl, Pamela A Silver and Edwin H Wintermute

    Citation: Journal of Biological Engineering 2011 5:7

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  7. Content type: Review

    Microfluidics holds great promise to revolutionize various areas of biological engineering, such as single cell analysis, environmental monitoring, regenerative medicine, and point-of-care diagnostics. Despite...

    Authors: Mandy LY Sin, Jian Gao, Joseph C Liao and Pak Kin Wong

    Citation: Journal of Biological Engineering 2011 5:6

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  8. Content type: Research

    An investigation of the electrochemical activity of human white blood cells (WBC) for biofuel cell (BFC) applications is described. WBCs isolated from whole human blood were suspended in PBS and introduced int...

    Authors: Gusphyl A Justin, Yingze Zhang, X Tracy Cui, Charles W Bradberry, Mingui Sun and Robert J Sclabassi

    Citation: Journal of Biological Engineering 2011 5:5

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  9. Content type: Review

    The U.S. National Academy of Engineering (NAE) recently published a document presenting "Grand Challenges for Engineering". This list was proposed by leading engineers and scientists from around the world at t...

    Authors: Mark R Riley, Charles P Gerba and Menachem Elimelech

    Citation: Journal of Biological Engineering 2011 5:2

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  10. Content type: Research

    Dendritic cells (DCs) are antigen-presenting immune cells that interact with T cells and have been widely studied for vaccine applications. To achieve this, DCs can be manipulated by lentiviral vectors (LVs) t...

    Authors: April Tai, Steven Froelich, Kye-Il Joo and Pin Wang

    Citation: Journal of Biological Engineering 2011 5:1

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  11. Content type: Methodology

    BioBrick standard biological parts are designed to make biological systems easier to engineer (e.g. assemble, manipulate, and modify). There are over 5,000 parts available in the Registry of Standard Biologica...

    Authors: Julie E Norville, Ratmir Derda, Saurabh Gupta, Kelly A Drinkwater, Angela M Belcher, Andres E Leschziner and Thomas F Knight Jr

    Citation: Journal of Biological Engineering 2010 4:17

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  12. Content type: Research

    In synthetic biology, gene regulatory circuits are often constructed by combining smaller circuit components. Connections between components are achieved by transcription factors acting on promoters. If the in...

    Authors: Kyung H Kim and Herbert M Sauro

    Citation: Journal of Biological Engineering 2010 4:16

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  13. Content type: Review

    For years, the field of drug delivery has focused on (1) controlling the release of a therapeutic and (2) targeting the therapeutic to a specific cell type. These research endeavors have concentrated mainly on...

    Authors: Jin-Oh You, Dariela Almeda, George JC Ye and Debra T Auguste

    Citation: Journal of Biological Engineering 2010 4:15

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  14. Content type: Research

    Most of synthetic circuits developed so far have been designed by an ad hoc approach, using a small number of components (i.e. LacI, TetR) and a trial and error strategy. We are at the point where an increasin...

    Authors: Francesca Ceroni, Simone Furini, Emanuele Giordano and Silvio Cavalcanti

    Citation: Journal of Biological Engineering 2010 4:14

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  15. Content type: Research

    One problem with engineered genetic circuits in synthetic microbes is their stability over evolutionary time in the absence of selective pressure. Since design of a selective environment for maintaining functi...

    Authors: Sean C Sleight, Bryan A Bartley, Jane A Lieviant and Herbert M Sauro

    Citation: Journal of Biological Engineering 2010 4:12

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  16. Content type: Review

    Molecular rotors are a group of fluorescent molecules that form twisted intramolecular charge transfer (TICT) states upon photoexcitation. When intramolecular twisting occurs, the molecular rotor returns to th...

    Authors: Mark A Haidekker and Emmanuel A Theodorakis

    Citation: Journal of Biological Engineering 2010 4:11

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  17. Content type: Research

    Membrane proteins are an important class of proteins, playing a key role in many biological processes, and are a promising target in pharmaceutical development. However, membrane proteins are often difficult t...

    Authors: Karan Bansal, Ke Yang, Goutam J Nistala, Robert B Gennis and Kaustubh D Bhalerao

    Citation: Journal of Biological Engineering 2010 4:6

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  18. Content type: Research

    A polarized light scattering technique was used to monitor the performance of a continuously operated foam fractionation process. The S11 and S12 parameters, elements of the light scattering matrix, combined toge...

    Authors: Janakiraman N Swamy, Czarena L Crofcheck and MP Mengüç

    Citation: Journal of Biological Engineering 2010 4:5

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  19. Content type: Research

    Positive feedback is a common mechanism used in the regulation of many gene circuits as it can amplify the response to inducers and also generate binary outputs and hysteresis. In the context of electrical cir...

    Authors: Goutam J Nistala, Kang Wu, Christopher V Rao and Kaustubh D Bhalerao

    Citation: Journal of Biological Engineering 2010 4:4

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  20. Content type: Research

    The engineering of metabolism holds tremendous promise for the production of desirable metabolites, particularly alternative fuels and other highly reduced molecules. Engineering approaches must redirect the t...

    Authors: Christina M Agapakis, Daniel C Ducat, Patrick M Boyle, Edwin H Wintermute, Jeffrey C Way and Pamela A Silver

    Citation: Journal of Biological Engineering 2010 4:3

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  21. Content type: Research

    The highly reactive aldehyde acrolein is a very potent endogenous toxin with a long half-life. Acrolein is produced within cells after insult, and is a central player in slow and progressive "secondary injury"...

    Authors: Youngnam Cho, Riyi Shi and Richard Ben Borgens

    Citation: Journal of Biological Engineering 2010 4:2

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  22. Content type: Methodology

    Standard biological parts, such as BioBricks™ parts, provide the foundation for a new engineering discipline that enables the design and construction of synthetic biological systems with a variety of applicati...

    Authors: JChristopher Anderson, John E Dueber, Mariana Leguia, Gabriel C Wu, Jonathan A Goler, Adam P Arkin and Jay D Keasling

    Citation: Journal of Biological Engineering 2010 4:1

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  23. Content type: Research

    Synthetic biology brings together concepts and techniques from engineering and biology. In this field, computer-aided design (CAD) is necessary in order to bridge the gap between computational modeling and bio...

    Authors: Deepak Chandran, Frank T Bergmann and Herbert M Sauro

    Citation: Journal of Biological Engineering 2009 3:19

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  24. Content type: Research

    Efflux pump activity has been associated with multidrug resistance phenotypes in bacteria, compromising the effectiveness of antimicrobial therapy. The development of methods for the early detection and quanti...

    Authors: Laura Paixão, Liliana Rodrigues, Isabel Couto, Marta Martins, Pedro Fernandes, Carla CCR de Carvalho, Gabriel A Monteiro, Filipe Sansonetty, Leonard Amaral and Miguel Viveiros

    Citation: Journal of Biological Engineering 2009 3:18

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  25. Content type: Research

    Cnidocysts isolated from cnidarian organisms are attractive as a drug-delivery platform due to their fast, efficient delivery of toxins. The cnidocyst could be utilized as the means to deliver therapeutics in ...

    Authors: Shawn C Oppegard, Peter A Anderson and David T Eddington

    Citation: Journal of Biological Engineering 2009 3:17

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  26. Content type: Research

    Biological engineering will play a significant role in solving many of the world's problems in medicine, agriculture, and the environment. Recently the U.S. National Academy of Engineering (NAE) released a doc...

    Authors: Jeong-Yeol Yoon and Mark R Riley

    Citation: Journal of Biological Engineering 2009 3:16

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  27. Content type: Research

    Advances in synthetic biology will require spatio-temporal regulation of biological processes in heterologous host cells. We develop a light-switchable, two-hybrid interaction in yeast, based upon the Arabidop...

    Authors: Oxana Sorokina, Anita Kapus, Kata Terecskei, Laura E Dixon, Laszlo Kozma-Bognar, Ferenc Nagy and Andrew J Millar

    Citation: Journal of Biological Engineering 2009 3:15

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  28. Content type: Research

    Determination of lipophilicity as a tool for predicting pharmacokinetic molecular behavior is limited by the predictive power of available experimental models of the biomembrane. There is current interest, the...

    Authors: Sunday Olakunle Idowu, Morenikeji Ambali Adeyemo and Udochi Ihechiluru Ogbonna

    Citation: Journal of Biological Engineering 2009 3:14

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  29. Content type: Research

    Escherichia coli exhibits diauxic growth in sugar mixtures due to CRP-mediated catabolite repression and inducer exclusion related to phosphotransferase system enzyme activity. Replacement of the native crp gene ...

    Authors: Reza Khankal, Jonathan W Chin, Debashis Ghosh and Patrick C Cirino

    Citation: Journal of Biological Engineering 2009 3:13

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  30. Content type: Research

    Bacterial cellulose has been used in the food industry for applications such as low-calorie desserts, salads, and fabricated foods. It has also been used in the paper manufacturing industry to enhance paper st...

    Authors: Kuan-Chen Cheng, Jeff M Catchmark and Ali Demirci

    Citation: Journal of Biological Engineering 2009 3:12

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  31. Content type: Research

    The Hamiltonian Path Problem asks whether there is a route in a directed graph from a beginning node to an ending node, visiting each node exactly once. The Hamiltonian Path Problem is NP complete, achieving s...

    Authors: Jordan Baumgardner, Karen Acker, Oyinade Adefuye, Samuel Thomas Crowley, Will DeLoache, James O Dickson, Lane Heard, Andrew T Martens, Nickolaus Morton, Michelle Ritter, Amber Shoecraft, Jessica Treece, Matthew Unzicker, Amanda Valencia, Mike Waters, AMalcolm Campbell…

    Citation: Journal of Biological Engineering 2009 3:11

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  32. Content type: Research

    Gradients of morphogens pattern cell fate – a phenomenon that is especially important during development. A simple model system for studying how morphogens pattern cell behavior would overcome difficulties inh...

    Authors: Takayuki Sohka, Richard A Heins and Marc Ostermeier

    Citation: Journal of Biological Engineering 2009 3:10

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  33. Content type: Research

    The release of heavy metal-containing nanoparticles (NP) into the environment may be harmful to the efficacy of beneficial microbes that function in element cycling, pollutant degradation and plant growth. Nan...

    Authors: Priyanka Gajjar, Brian Pettee, David W Britt, Wenjie Huang, William P Johnson and Anne J Anderson

    Citation: Journal of Biological Engineering 2009 3:9

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  34. Content type: Research

    The following essay was written by a sophomore undergraduate student majoring in Bioengineering at the University of Maryland, Mr. Zachary Russ. Mr. Russ was one of 174 students who submitted a 1000–1200 word ...

    Authors: Zachary N Russ

    Citation: Journal of Biological Engineering 2009 3:7

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  35. Content type: Research

    Self-assembly of the amyloid-β peptide (Aβ) has been implicated in the pathogenesis of Alzheimer's disease (AD). As a result, synthetic molecules capable of inhibiting Aβ self-assembly could serve as therapeut...

    Authors: Adriana A Reyes Barcelo, Francisco J Gonzalez-Velasquez and Melissa A Moss

    Citation: Journal of Biological Engineering 2009 3:5

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  36. Content type: Methodology

    The engineering of many-component, synthetic biological systems is being made easier by the development of collections of reusable, standard biological parts. However, the complexity of biology makes it diffic...

    Authors: Jason R Kelly, Adam J Rubin, Joseph H Davis, Caroline M Ajo-Franklin, John Cumbers, Michael J Czar, Kim de Mora, Aaron L Glieberman, Dileep D Monie and Drew Endy

    Citation: Journal of Biological Engineering 2009 3:4

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  37. Content type: Research

    Apple juice prepared from 'Annurca' apple puree was treated with a HPCD batch system. The pH, °Brix, color parameters and microbial load of the treated apple juice were compared with those of thermally process...

    Authors: Giovanna Ferrentino, Mariacarmela Bruno, Giovanna Ferrari, Massimo Poletto and Murat O Balaban

    Citation: Journal of Biological Engineering 2009 3:3

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  38. Content type: Research

    The current knowledge of genes and proteins comes from 'naturally designed' coding and non-coding regions. It would be interesting to move beyond natural boundaries and make user-defined parts. To explore this...

    Authors: Pawan K Dhar, Chaw Su Thwin, Kyaw Tun, Yuko Tsumoto, Sebastian Maurer-Stroh, Frank Eisenhaber and Uttam Surana

    Citation: Journal of Biological Engineering 2009 3:2

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  39. Content type: Research

    Microbial communities are involved in many processes relevant to industrial and medical biotechnology, such as the formation of biofilms, lignocellulosic degradation, and hydrogen production. The manipulation ...

    Authors: Travis S Bayer, Kevin G Hoff, Chase L Beisel, Jack J Lee and Christina D Smolke

    Citation: Journal of Biological Engineering 2009 3:1

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  40. Content type: Research

    Immediately after damage to the nervous system, a cascade of physical, physiological, and anatomical events lead to the collapse of neuronal function and often death. This progression of injury processes is ca...

    Authors: Mahvash Zuberi, Peishan Liu-Snyder, Aeraj ul Haque, David M Porterfield and Richard B Borgens

    Citation: Journal of Biological Engineering 2008 2:17

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