Figure 2

Light switchable light output. Yeast cells harboring the GAL1:LUC reporter and expressing PHYA-GBD/GAD-FHY1 (A), PHYA-GBD/GAD-FHL (B), or PHYBNT-GBD/GAD-PIF3 (C) fusion protein-pairs were grown in darkness to form patches (merged colonies) for two days at 30°C, treated with 2.5 mM luciferin and transferred to 22°C for 17.5 h. Separate yeast patches were irradiated with single red (R), or far-red (FR) light pulses, or with red pulses immediately followed by far-red pulses (R/FR), or were kept in darkness (Dark). Luminescence values normalised to the pre-pulse levels are shown; time 0 h is the start of the light treatment. The luciferin pretreatment is shown in Additional file 1. E: Selected luminescent images of yeast patches used to obtain data in panel A. I: red light-induced, NI: non-induced dark control, T0: last images before the light pulse. Consecutive luminescent images taken in every two hours are shown. Pictures are displayed in pseudo-colors: red-white or blue-black colors indicate high or low expression levels, respectively.