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Figure 5 | Journal of Biological Engineering

Figure 5

From: Evolutionary principles and synthetic biology: avoiding a molecular tragedy of the commons with an engineered phage

Figure 5

Separate amplification on selective hosts. Amplifications of T70 and T7E1 on the capsulated host (EV36 in M9) when grown in physical isolation of each other. There is a superiority of T7E1 at all initial phage densities tested, although the difference is (highly) statistically significant at only the 3 highest densities in individual, 2-tailed t-tests (tests were performed on differences between each pair of T70 and T7E1 samples most similar in initial density). This assay demonstrates that the endosialidase protein enhances phage growth on the capsulated host. Amplifications here are lower than in Figure 4 because the host and media used here (M9 glucose) are not as conducive to cell growth. Assays were paired, so that T7E1 and T70 amplification samples were taken from different zones on the same plates. Bars span 2 standard errors.

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