Figure 3

BioBrick miraculin and brazzein protein expression in bacteria and yeast. (A) Miraculin and (B) brazzein BioBricks were expressed from an IPTG-inducible promoter in E. coli with an N- or C-terminal Strep-II tag. Miraculin was expressed at low levels, with only a faint band appearing at 24 kDa. Brazzein was well expressed in an IPTG-dependent manner. (C) Brazzein BioBrick was expressed in yeast from pTEF or pCup1 promoters with a C- terminal Strep-II tag. Brazzein appeared larger in yeast vs. E. coli, most likely due to glycosylation of brazzein in yeast.