Figure 2
From: Targeting a heterologous protein to multiple plant organelles via rationally designed 5′ mRNA tags
Design of TriTag-3, containing peroxisome-targeting TTL tag embedded within the chloroplast-targeting rbcS1 tag. (a, b) Diagrams of potato Rubisco rbcS1 chloroplast targeting tag CTPb (a) and TriTag-3 (b), showing chloroplast targeting sequences (light green), peroxisome targeting sequences (tan), flexible regions (diagonals), and the enhanced GFP coding sequence used in transient expression experiments. (c, d) CTPb (c) and TriTag-3 (d) sequences. The ATG codon at the end corresponds to the first residue of the GFP open reading frame. The light green DNA sequences derive from the rbc S1 5′ coding region [1] and encode a chloroplast targeting sequence (green). The light tan DNA sequences (d) code for a consensus PTS2 signal (tan). The PTS2 sequence is embedded within a flexible region of CTPb (green diagonals).