Figure 2
From: In silico design and in vivoimplementation of yeast gene Boolean gates
Single-input gates. A) Tetracycline responsive YES gates. B) β-estradiol sensing NOT gates. In both cases, a single operator guarantees higher signal separation whereas two operators permit to achieve a stronger repression. Fluorescence levels of the open gates (indicated with their yeast strain name–see Additional file 1) correspond to blue columns. Gates’ schemes are drawn with SBOL [36] icons (all the symbols used throughout the paper are shown in Additional file 1: Figure S1).