Table 2 The applications of nucleofection in related studies
From: Nucleic acid direct delivery to fibroblasts: a review of nucleofection and applications
Application purpose | Experi-mental animal | Target cell | Transferred gene | Cell administration | Result | Reference |
|---|---|---|---|---|---|---|
Treatment of lower extremity ischemia | Rat | Rat dermal fibroblasts | ·VEGF165·bFGF | Local injection | Enhanced collateral formation in ischemic areas | [11] |
Research of Alport syndrome | In vitro | Human dermal fibroblasts | ·OCT3/4·SOX2·KLF4·LIN28·L-Myc·p53 shRNA | Cultured in vitro | Generation of colonies of induced pluripotent stem cells | |
Research of systemic sclerosis | In vitro | Human dermal fibroblasts | ·c-Jun siRNA·c-Fos siRNA | Cultured in vitro | Identification of c-Jun and c-Fos as TGFβ downstream mediators of the profibrotic effect in systemic sclerosis | [25] |
Swine embryo engineering | In vitro | Porcine fetal fibroblasts | ·Human growth hormone gene·Rat whey acidic protein promoter | Embryo reconstitution in vitro | Improved embryonic development | [29] |
Research of pig-to-primate xenotransplantati-on | In vitro | Porcine fetal fibroblasts | ·α 1,3-galactosyltra-nsferase (GalT) (KO)·Membrane cofactor protein (MCP) | Human serum-mediated cytolysis assay | Reduced cytolysis | [30] |
Treatment of sensorineural hearing loss | In vitro | Rat sciatic nerve fibroblasts | ·Brain-derived neurotrophic factor (BDNF)·Neurotrophic factor 3 (NT3) | Cultured in vitro | ·Successful transfection and expressio of BDNF and NT3·Survival maintenance of spiral ganglion neurons | [31] |
Improvement of angiogenesis in ischemic flap | Rat | Rat dermal fibroblasts | VEGF164 | Local subcutaneous injection in the flap area | Acceleration of neovasculariza-tion | [33] |
Production of human insulin using transgenic buffaloes | In vitro | Buffalo fetal fibroblasts | Expression vector for the human insulin gene inserted between the DNA fragments of the promoter and terminator regions of mammary gland-specific buffalo β-lactoglobulin | Embryo reconstitution in vitro | ·Successful human insulin gene transfection·Reduced embryonic development | |
Research and treatment of neurodegenerative diseases | In vitro | Human foreskin fibroblasts | ·Ascl1·miRNA124·p53 shRNA | Cultured with neuronal induction and maturation medium | Successful conversion of human fibroblasts into neurons | [50] |
Research of Friedreich's ataxia (FRDA) | In vitro | FRDA patient fibroblasts | pCR3.1-TALEFrat#8-TADs | Cultured in vitro | Increased frataxin protein expression and maturation levels | [51] |
Research of equine sarcoid | In vitro | Equine adult cutaneous fibroblast cells (ACFCs) | BPV-E4/ BPV-E1^E4 | Cultured in vitro | Sarcoid-like neoplastic transformation of equine ACFCs | [56] |