Fig. 1
From: Mixed culture biocatalytic production of the high-value biochemical 7-methylxanthine
Conversion of caffeine to 7-methylxanthine occurs via theobromine when N-demethylation is carried out sequentially by cells expressing the wild-type ndmA (blue) and ndmB (red) genes, and by paraxanthine when N-demethylation is carried out by E. coli strain MBM019 expressing the mutant ndmA4 gene (green). Price of methylxanthines per gram shown is based on lowest retail values from Sigma-Aldrich as of August 2022