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Table 1 Flux distribution indicated by different engineering approaches in different strains to enhance 2,3-BD production (mmol/gCDW/h)a

From: Investigation of two metabolic engineering approaches for (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis

Method

Single-level FBA

OptKnockb

B. subtilis strain

W168

LM01c

W168

Biomass production

0.30

0.28

0.35

Acetate exchange

3.78

0.12

0.15

l-Lactate exchange

2.00

0.00

0.00

(R,R)-2,3-BD exchange

0.00

2.54

2.10

  1. aGlycerol utilization and oxygen consumption were set to 10 mmol/gCDW/h (see Methods)
  2. bOptKnock objectives were set for growth coupling with 2,3-BD production. The reactions suggested for gene knockout were: ACKr (ack), PTAr (pta), ACACT1r (mmgA), and G6PDH2r (zwf). This informed the construction of MZ02 strain
  3. cLM01 was designed to be unable to perform four reactions, namely, ACKr (ack), PTAr (pta), LDH_L (lctE), and ACACT1r (mmgA)
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Journal of Biological Engineering

ISSN: 1754-1611