Investigation of two metabolic engineering approaches for (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis

Table 2 Major VFA yields and glycerol utilization during B. subtilis growth

Strain	VFA (mol/mol‧glycerol) at 0–24 h				Total glycerol utilization (mmol)
Strain	Acetate	Lactate	Acetoin	2,3-BD	Total glycerol utilization (mmol)
W168	0.27 ± 0.02	0.17 ± 0.00	0.01 ± 0.01	0.00 ± 0.00	13.4 ± 0.37
LM01	0.00 ± 0.00	0.01 ± 0.01	0.00 ± 0.00	0.03 ± 0.05	8.4 ± 0.16
MZ02	0.03 ± 0.03	0.21 ± 0.06	0.00 ± 0.00	0.00 ± 0.00	10.6 ± 1.64
Strain	VFA (mol/mol‧glycerol) at 24–36 h				Total glycerol utilization (mmol)
Strain	Acetate	Lactate	Acetoin	2,3-BD	Total glycerol utilization (mmol)
W168	0.00 ± 0.00	0.00 ± 0.00	0.01 ± 0.01	0.03 ± 0.02	9.7 ± 3.75
LM01	0.12 ± 0.02	0.00 ± 0.00	0.00 ± 0.01	0.05 ± 0.00	11.7 ± 1.79
MZ02	0.09 ± 0.01	0.01 ± 0.01	0.05 ± 0.02	0.05 ± 0.01	13.1 ± 0.45
Strain	VFA (mol/mol‧glycerol) at 36–96 h				Total glycerol utilization (mmol)
Strain	Acetate	Lactate	Acetoin	2,3-BD	Total glycerol utilization (mmol)
W168	0.00 ± 0.00	0.00 ± 0.00	0.12 ± 0.00	0.16 ± 0.01	69.9 ± 1.10
LM01	0.02 ± 0.01	0.00 ± 0.00	0.03 ± 0.01	0.20 ± 0.01	63.8 ± 1.71
MZ02	0.00 ± 0.00	0.00 ± 0.00	0.05 ± 0.00	0.14 ± 0.01	69.6 ± 3.29

ISSN: 1754-1611