Table 5 Strains and plasmids used in the current study

Strains

 E. coli Nova blue

endA1 hsdR17(r_K12⁻m_K12⁺) supE44 thi-I gyrA96 relA1 lac recA1/F’[proAB + lacIq ZΔM15::Tn10(Tetr)]

Novagen

 B. subtilis W168

trpC2

JMC^a

 B. subtilis LM01

trpC2 ∆ackA ∆pta ∆lctE ∆mmgA

[7]

 B. subtilis MZ02

trpC2 ∆ackA ∆pta ∆mmgA ∆zwf

This study

Plasmids

 pJOE8999

Kan^R, P_manPA-cas9, P_vanP*, lacPOZ’-gRNA, oop ter, T7P, repE194^ts, pUCori

[34]

 pJOE_ackA

pJOE8999 derivative harboring 20-nt spacer for ackA gene targeting and homologous arms for fusion

[7]

 pJOE_pta

pJOE8999 derivative harboring 20-nt spacer for pta gene targeting and homologous arms for fusion

[7]

 pJOE_mmgA

pJOE8999 derivative harboring 20-nt spacer for mmgA gene targeting and homologous arms for fusion

[7]

 pJOE_zwf

pJOE8999 derivative harboring 20-nt spacer for zwf gene targeting and homologous arms for fusion

This study