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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: The synergistic and enhancive effects of IL-6 and M-CSF to expand and differentiate functional dendritic cells from human monocytes under serum-free condition

Fig. 1

CD40 and CD209 profiles, growth kinetics and morphologies on DC differentiation from human monocytes. DCs were differentiated from CD14+ monocytes (5 × 105 cells/1.5 mL) in SF-DC Optimal medium, SF-DC Control medium and SF-DC Control + Serum medium for 5 days. A The differentiated cells in SF-DC Optimal medium at the indicated time points were stained with CD40 and CD209-PE. The numbers within the dot plots represent the percentages of the indicated cells in the total cell population by flow cytometry analysis. B The accumulated total cell number, percentage of CD40+CD209+ DCs in the total cells and CD40+CD209+ DC number differentiated from CD14+ monocytes at the indicated time points were determined by flow cytometry analysis (n = 5). (I) Representative morphologies of the differentiated DCs on Day 5 in SF-DC Optimal medium under scanning electron microscopy observation. Scale bars are 10 μm (left figure) and 5 μm (right figure)

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Journal of Biological Engineering

ISSN: 1754-1611