Fig. 2
Representative histograms of DC-related surface marker expressions on differentiated DCs from human monocytes in SF-DC Optimal medium. DCs were differentiated from CD14+ monocytes (5 × 105 cells/1.5 mL) in SF-DC Optimal medium for 5 days (Day 0 to Day 5), and then, 20 ng/ml TNF-α or 1 μg/mL lipopolysaccharide (LPS) was added to stimulate maturation for 2 days (Day 5 to Day 7). After differentiation, the generated cells at the indicated time points (Day 0: black peaks; Day 5: red peaks; Day 7 with TNF-α: blue peaks; Day 7 with LPS: green peaks) were stained with antibodies against (A) CD40, B CD209, C CD1a, D CD11c, E CD14, F CD80, G CD83 and H CD86. The histogram of the indicated surface marker expression in the total cell population was analyzed by flow cytometry