Fig. 3
Expression of DC-related surface markers on differentiated DCs from human monocytes. DCs were differentiated from CD14+ monocytes (5 × 105 cells/1.5 mL) in SF-DC Optimal medium, SF-DC Control medium and SF-DC Control + Serum medium for 5 days (Day 0 to Day 5), and then, 20 ng/ml TNF-α was added to the corresponding medium to stimulate maturation for 2 days (Day 5 to Day 7). After differentiation, the generated cells at the indicated time points were stained with antibodies against (A) CD40, B CD209, C CD1a, D CD11c, E CD14, F CD80, G CD83 and H CD86. The mean fluorescence intensity (MFI) of the indicated cells in the total cell population was analyzed by flow cytometry. *, ** and *** represent significant differences of p < 0.05, p < 0.01 and p < 0.005, respectively (n = 5)