Fig. 4
Comparison of DC-related surface marker expression on differentiated DCs from human monocytes in SF-DC Optimal medium with TNF-α and lipopolysaccharide stimulation. DCs were differentiated from CD14+ monocytes (5 × 105 cells/1.5 mL) in SF-DC Optimal medium for 5 days (Day 0 to Day 5), and then, 20 ng/ml TNF-α or 1 μg/mL lipopolysaccharide (LPS) was added to stimulate maturation for 2 days (Day 5 to Day 7). After differentiation, the generated cells on Day 7 were stained with antibodies against (A) CD40, B CD209, C CD1a, D CD11c, E CD14, F CD80, G CD83 and (H) CD86. The mean fluorescence intensity (MFI) of the indicated cells in the total cell population was analyzed by flow cytometry. *, ** and *** represent significant differences of p < 0.05, p < 0.01 and p < 0.005, respectively (n = 5)