Fig. 5
Endocytosis analysis of the differentiated DCs from monocytes. DCs were differentiated from CD14+ monocytes (5 × 105 cells/1.5 mL) in SF-DC Optimal medium, SF-DC Control medium and SF-DC Control + Serum medium for 5 days (Day 0), and then, 20 ng/ml TNF-α or 1 μg/mL lipopolysaccharide (LPS) was added to the corresponding medium to stimulate maturation for 2 days (Day 5 to Day 7). After differentiation, the cells were treated with 1 mg/mL dextran-fluorescein isothiocyanate (dextran-FITC) or 1 mg/mL fluorescent latex beads and stained with a CD209 fluorescence antibody. The endocytosis ability was determined by the CD209+ cells that expressed the fluorescence of dextran-FITC or latex beads using flow cytometry. A Representative endocytosis analysis of the differentiated DCs in SF-DC Optimal medium before (Day 5) and after (Day 7) TNF-α or LPS stimulation for maturation by flow cytometry. B The percentages and the mean fluorescence intensity (MFI) of the cells with dextran-FITC or fluorescent latex bead uptake in total CD209+ DCs at the indicated time points were analyzed by flow cytometry. ** and *** represent significant differences of p < 0.01 and p < 0.005, respectively (n = 3)