Fig. 7
Cytokine secretion of the differentiated DCs from monocytes. DCs were differentiated from CD14+ monocytes (5 × 105 cells/1.5 mL) in SF-DC Optimal medium, SF-DC Control medium and SF-DC Control + Serum medium for 5 days (Day 0 to Day 5), and then, 20 ng/ml TNF-α was added to the corresponding medium to stimulate maturation for 2 days (Day 5 to Day 7). After differentiation, the conditioned media at the indicated time points were collected, and the secreted amounts of (A) IL-1β, B IL-8, C IL-10 and D IL-12p70 were analyzed by flow cytometry. *, ** and *** represent significant differences of p < 0.05, p < 0.01 and p < 0.005, respectively (n = 3)