Table 1 Matrix of the 24 full factorial design and experimental results of cytokine screening for CD40+CD209+ cells differentiated from CD14+ monocytes in the SF-DC Control mediuma,b
Trial | IL-1β | IL-6 | IL-7 | M-CSF | Total cells (×104 cells)c | CD40+CD209+ (%)c | CD40+CD209+ cells (×104 cells)c |
|---|---|---|---|---|---|---|---|
1 | -1 | -1 | -1 | -1 | 3.8 ± 0.7 | 40.8 ± 5.0% | 1.6 ± 0.3 |
2 | + 1 | -1 | -1 | -1 | 4.8 ± 0.9 | 68.3 ± 1.8% | 3.3 ± 0.4 |
3 | -1 | + 1 | -1 | -1 | 5.7 ± 0.9 | 62.9 ± 1.3% | 3.6 ± 0.5 |
4 | + 1 | + 1 | -1 | -1 | 3.6 ± 0.1 | 69.1 ± 2.9% | 2.5 ± 0.1 |
5 | -1 | -1 | + 1 | -1 | 3.8 ± 0.4 | 43.3 ± 5.5% | 1.7 ± 0.3 |
6 | + 1 | -1 | + 1 | -1 | 3.3 ± 0.6 | 73.1 ± 2.4% | 2.5 ± 0.3 |
7 | -1 | + 1 | + 1 | -1 | 5.7 ± 1.2 | 67.0 ± 1.4% | 3.8 ± 0.4 |
8 | + 1 | + 1 | + 1 | -1 | 3.4 ± 0.7 | 69.2 ± 2.7% | 2.4 ± 0.3 |
9 | -1 | -1 | -1 | + 1 | 5.2 ± 0.9 | 85.5 ± 1.0% | 4.5 ± 0.4 |
10 | + 1 | -1 | -1 | + 1 | 3.2 ± 0.2 | 66.2 ± 2.7% | 2.2 ± 0.2 |
11 | -1 | + 1 | -1 | + 1 | 5.4 ± 0.7 | 76.1 ± 2.1% | 4.2 ± 0.4 |
12 | + 1 | + 1 | -1 | + 1 | 3.7 ± 0.5 | 65.1 ± 2.0% | 2.4 ± 0.3 |
13 | -1 | -1 | + 1 | + 1 | 5.3 ± 0.8 | 84.0 ± 2.2% | 4.6 ± 0.5 |
14 | + 1 | -1 | + 1 | + 1 | 3.6 ± 0.6 | 65.7 ± 2.1% | 2.4 ± 0.3 |
15 | -1 | + 1 | + 1 | + 1 | 4.0 ± 0.6 | 75.6 ± 1.7% | 3.1 ± 0.3 |
16 | + 1 | + 1 | + 1 | + 1 | 3.3 ± 0.3 | 64.2 ± 2.0% | 2.2 ± 0.2 |