The synergistic and enhancive effects of IL-6 and M-CSF to expand and differentiate functional dendritic cells from human monocytes under serum-free condition

Table 2 The concentrations of cytokines along the steepest ascent path and the numbers of CD40⁺CD209⁺ DCs differentiated from CD14⁺ monocytes^a

Step	IL-6 (ng/ml)	M-CSF (ng/ml)	GM-CSF (ng/ml)	IL-4 (ng/ml)	Total cells (10⁴ cells)^b	CD40⁺CD209⁺ (%) ^b	CD40⁺CD209⁺ cells (10⁴ cells)^b
1	0	0	50.0	50.0	4.1 ± 0.4	38.7 ± 6.1%	1.6 ± 0.1
2	0.5	1.0	50.0	50.0	6.8 ± 0.7	57.8 ± 1.2%	3.9 ± 0.6
3	0.9	2.0	50.0	50.0	6.9 ± 0.8	70.6 ± 2.0%	4.9 ± 0.8
4	1.4	3.0	50.0	50.0	7.2 ± 1.0	74.5 ± 1.0%	5.4 ± 0.7
5	1.9	4.0	50.0	50.0	7.1 ± 1.1	74.4 ± 2.9%	5.3 ± 0.6
6	2.3	5.0	50.0	50.0	6.8 ± 1.3	74.1 ± 0.5%	5.0 ± 0.9
7	2.8	6.0	50.0	50.0	7.0 ± 1.0	74.9 ± 2.1%	5.2 ± 0.9
8	3.3	7.0	50.0	50.0	6.9 ± 0.9	77.4 ± 2.2%	5.3 ± 0.8
9	3.7	8.0	50.0	50.0	6.8 ± 0.8	74.0 ± 2.7%	5.1 ± 0.9
10	4.2	9.0	50.0	50.0	6.3 ± 0.8	74.8 ± 2.2%	4.7 ± 0.8
11	4.7	10.0	50.0	50.0	6.4 ± 0.8	76.6 ± 2.8%	4.9 ± 0.7
12	5.9	12.5	50.0	50.0	6.6 ± 1.0	73.6 ± 2.2%	4.9 ± 0.7
13	7.0	15.0	50.0	50.0	6.6 ± 0.8	73.5 ± 2.0%	4.8 ± 0.8
14	9.4	20.0	50.0	50.0	6.7 ± 0.8	73.2 ± 2.8%	4.9 ± 1.0

^aThe initial cell seeding density was 5 × 10⁵ CD14⁺ cells in 1.5 ml differentiation medium
^bCell numbers in 1.5 ml differentiation medium and CD40⁺CD209⁺ percentage at day 5 (n = 3)

ISSN: 1754-1611