Fig. 2
From: Synergistic improvement of cinnamylamine production by metabolic regulation
Effects of five global TFs on the conversion of cinnamic acid to cinnamylamine. The reaction was performed in M9 buffer (pH 7.0) containing 10 mM cinnamic acid (the initial concentration); 20 mM L-Ala; 20 mM MgSO4; S003-S009 wet cells, OD600 nm = 30 at 30 °C with 200 rpm shaking for 1 h. Not recovered: undetectable cinnamic acid. It may be converted to other unknown byproducts. According to our previous report [17], the by-product pyruvate generated during the reaction can replace glucose as the carbon source for the reaction (Scheme 1)