Table 1 Plasmids or strains used in this study
From: Synergistic improvement of cinnamylamine production by metabolic regulation
No. | Plasmids or strains | Description | Source |
|---|---|---|---|
p001 | pET28a-nccar-pptase | T7, nccar with His tag, T7, pptase, lacIq, pBR322 ori, Kanr | Laboratory stock |
p002 | pACYCDuet1-oata | T7, oata, lacIq, pBR322 ori, Cmr | Laboratory stock |
p003 | pACYCDuet1-oata-arcA | T7, oata, T7, arcA, lacIq, pBR322 ori, Cmr | This study |
p004 | pACYCDuet1-oata-cra | T7, oata, T7, cra, lacIq, pBR322 ori, Cmr | This study |
p005 | pACYCDuet1-oata-crp | T7, oata, T7, crp, lacIq, pBR322 ori, Cmr | This study |
p006 | pACYCDuet1-oata-fnr | T7, oata, T7, fnr, lacIq, pBR322 ori, Cmr | This study |
p007 | pACYCDuet1-oata-csrA | T7, oata, T7, csrA, lacIq, pBR322 ori, Cmr | This study |
p008 | pACYCDuet1-oata-marA | T7, oata, T7, marA, lacIq, pBR322 ori, Cmr | This study |
p009 | pACYCDuet1-oata-rob | T7, oata, T7, rob, lacIq, pBR322 ori, Cmr | This study |
p010 | pACYCDuet1-oata-soxS | T7, oata, T7, soxS, lacIq, pBR322 ori, Cmr | This study |
p011 | pACYCDuet1-oata-csgD | T7, oata, T7, csgD, lacIq, pBR322 ori, Cmr | This study |
p012 | pACYCDuet1-oata-emrE | T7, oata, T7, emrE, lacIq, pBR322 ori, Cmr | This study |
p013 | pACYCDuet1-oata-emrB | T7, oata, T7, emrB, lacIq, pBR322 ori, Cmr | This study |
p014 | pACYCDuet1-oata-rpoS | T7, oata, T7, rpoS, lacIq, pBR322 ori, Cmr | This study |
p015 | pACYCDuet1-oata-tolC | T7, oata, T7, tolC, lacIq, pBR322 ori, Cmr | This study |
p016 | pACYCDuet1-P1,6-oata | P1,6, oata, lacIq, pBR322 ori, Cmr | This study |
p017 | pACYCDuet1-P2,51-oata | P2,51, oata, lacIq, pBR322 ori, Cmr | This study |
p018 | pACYCDuet1-P1,6-oata-marA | P1,6, oata, T7, marA, lacIq, pBR322 ori, Cmr | This study |
S001 | E. coli RARE(DE3) | MG1655(DE3) ∆dkgB ∆yeaE ∆(yqhC-dkgA) ∆yahK ∆yjgB ∆yqhD | Addgene |
S002 | E. coli RARE(DE3) ∆arcA | MG1655(DE3) ∆dkgB ∆yeaE ∆(yqhC-dkgA) ∆yahK ∆yjgB ∆yqhD ∆arcA | Laboratory stock |
S003 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-oata | RARE(DE3) carrying p001 and p002 | This study |
S004 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-oata-arcA | RARE(DE3) carrying p001 and p003 | This study |
S005 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-oata-cra | RARE(DE3) carrying p001 and p004 | This study |
S006 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-oata-crp | RARE(DE3) carrying p001 and p005 | This study |
S007 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-oata-fnr | RARE(DE3) carrying p001 and p006 | This study |
S008 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-oata-csrA | RARE(DE3) carrying p001 and p007 | This study |
S009 | RARE(DE3)△arcA / pET28a-nccar-pptase/pACYCDuet1-oata | RARE(DE3)△arcA carrying p001 and p002 | This study |
S010 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-oata-marA | RARE(DE3) carrying p001 and p008 | This study |
S011 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-oata-rob | RARE(DE3) carrying p001 and p009 | This study |
S012 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-oata-soxS | RARE(DE3) carrying p001 and p010 | This study |
S013 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-oata-csgD | RARE(DE3) carrying p001 and p011 | This study |
S014 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-oata-emrE | RARE(DE3) carrying p001 and p012 | This study |
S015 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-oata-emrB | RARE(DE3) carrying p001 and p013 | This study |
S016 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-oata-rpoS | RARE(DE3) carrying p001 and p014 | This study |
S017 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-oata-tolC | RARE(DE3) carrying p001 and p015 | This study |
S018 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-P1,6-oata | RARE(DE3) carrying p001 and p016 | This study |
S019 | RARE(DE3)/ pET28a-nccar-pptase/pACYCDuet1-P2,51-oata | RARE(DE3) carrying p001 and p017 | This study |
S020 | RARE(DE3)△arcA / pET28a-nccar-pptase/pACYCDuet1-P1,6-oata | RARE(DE3)△arcA carrying p001 and p016 | This study |
S021 | RARE(DE3)△arcA / pET28a-nccar-pptase/pACYCDuet1-oata-marA | RARE(DE3)△arcA carrying p001 and p008 | This study |
S022 | RARE(DE3)△arcA / pET28a-nccar-pptase/pACYCDuet1-P1,6-oata-marA | RARE(DE3)△arcA carrying p001 and p018 | This study |