Fig. 9

The expression levels of the desired genes. Relative expression of (A) Oct-4, (B) Nanog, (C) TRX, (D) Sirt-1, (E) SOX2, and (F) GPX-1 in the released cell sheets by real-time PCR analysis; the high level of the expression in Oct-4, Nanog, TRX, SOX2, and GPX-1 and low level of the expression in Sirt-1 were found when treated with Vc and Se compared to the control media. The values were normalized to GAPDH. (*p < 0.05, **p < 0.01, and ***p < 0.001)