Experimental studies from shake flasks to 3 L stirred tank bioreactor of nutrients and oxygen supply conditions to improve the growth of the avian cell line DuckCelt®-T17

Table 1 Description of the different supplementation strategies studied at lab-scale in shake flasks with the corresponding DuckCelt®-T17 growth kinetic parameters. For each strategy, the supplemented basic culture medium is the OptiPRO™ SFM medium with antibiotics and Pluronic F68

Strategy	Description	µ_max (h^− 1)	t_D (h)	IVCC^a (10⁶ cells.d/mL)
A	Glutamine (4 mM, D0)	0.023	30.7	26.1 ± 1.1
B	Glutamax (4 mM, D0)	0.019	35.7	30.0 ± 1.5 (*)
C	Without glutamine	–	–	3.5 ± 0.2
D	Glutamine (4 mM, D0) + Glutamax (4 mM, D0)	0.016	43.1	23.0 ± 1.1
E	Glutamax (4 mM, D0) + Glutamax (4 mM, D0)	0.019	35.9	29.6 ± 1.1 (*)
F	Glutamine (4 mM, D0) + Glucose (D3, D6)	0.019	36.1	29.8 ± 0.7 (*)
G	Glutamax (D0) + Glucose (D3, D6)	0.018	39.2	27.8 ± 0.7
H	Glutamine (4 mM, D0) + Glutamax (4 mM, D3)	0.018	39.4	26.6 ± 0.7
I	Glutamine (2mM, D0) + Glutamine (2mM, D3)	0.019	37.3	31.4 ± 2.2 (*)
J	Glutamax (2mM, D0) + Glutamax (2mM, D3)	0.019	37.3	31.0 ± 1.7 (*)
K	Glutamine (4 mM, D0) + SFM (D3, D6, D9)	0.019	35.9	31.5 ± 0.4 (*)
L	Glutamine (4 mM, D0) + SFM w/o Gln (D3, D6, D9)	0.019	35.4	29.0 ± 0.6 (*)
M	Glutamax (4 mM, D0) + SFM (D3, D6, D9)	0.016	43.9	27.0 ± 0.7

^a Results are presented as mean ± SEM (n = 6), asterisk (*) indicates a p-value < 0.05 in t-test analysis

ISSN: 1754-1611