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Fig. 3 | Journal of Biological Engineering

Fig. 3

From: Dual growth factor-modified microspheres nesting human-derived umbilical cord mesenchymal stem cells for bone regeneration

Fig. 3

a A CCK8 assay was performed to evaluate the proliferation of the HUMSCs cocultured with each group of microspheres for 1, 3, and 7 days. One-way ANOVA with multiple comparison tests was used for statistical analysis (**p < 0.01). b Live/dead assay of HUMSCs cultured on each group of microspheres for 4 days. Green staining represented live cells, and red staining represented dead cells. Scale bar: 200 μm. c DAPI/phalloidin staining indicated the adhesion of HUMSCs on each group of microspheres for 4 days. Blue staining represents the cell nucleus, and red staining represents the cell cytoskeleton. Scale bar: 100 μm

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