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Fig. 6 | Journal of Biological Engineering

Fig. 6

From: Sandwich-like electro-conductive polyurethane-based gelatin/soybean oil nanofibrous scaffolds with a targeted release of simvastatin for cardiac tissue engineering

Fig. 6

Cellular studies. a FE-SEM micrographs of seeded H9C2 cells on electrospun nanofibers after 7 days of culture (scale bar: 50 μm). b H9C2 viability on electrospun nanofibers (****p < 0.0001). c Live-Dead staining images of H9C2 cells at 7 days after treatment with electrospun nanofibers (live: green, dead cells: red, scale bar:50 μm). d DAPI staining of rat H9C2 cells after 7 days of cell culture on electrospun nanofibers (scale bar:50 μm). Measuring protein levels of (e) α-actinin, Connexin-43, and myosin protein levels of the H9C2 cells on the nanofibrous scaffolds using western blotting. One-Way ANOVA with Tukey post hoc analysis. ****p < 0.0001

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