Fig. 2

Tm Evaluation of ColG and ColG-variant. Enzymes were incubated at temperatures ranging from 30 °C to 90 °C for one hour and cooled down to 25 °C prior to the collagenolytic activity assay. Residual collagenolytic activity was plotted as a function of temperature for ColG (Black) and ColG-variant (Red). The data represent the mean of three replicates ± standard deviation