Figure 3
From: A switchable light-input, light-output system modelled and constructed in yeast
Kinetics of induction of luciferase mRNA and luciferase activity from the CUP1:LUC reporter gene. Yeast cells harboring the CUP1:LUC construct were grown in liquid rich media overnight at 30°C. CUP1:LUC expression was induced by 1.5 mM copper-sulphate (final concentration) at time = 0 and aliquots were harvested hourly. The samples were used to prepare crude protein extracts and to isolate total RNA. Luciferase activity was measured by in vitro assays and luciferase mRNA was determined by qRT-PCR reactions.