Figure 3

Effect of chlorpromazine (CPZ) on EtBr accumulation by E. coli K-12 AG100. The bacteria were loaded with EtBr at 1 μg/ml in the presence of increasing concentrations of CPZ for a period of 60 minutes at 37°C in the presence and absence of glucose and accumulation assessed by: (A1 and A2) the semi-automated fluorometric method; (B) flow cytometry, represented by an histogram overlay corresponding to the data selected for analysis (2^nd gate) for E. coli K-12 AG100 (i) without EtBr or CPZ, (ii) after 60 minutes of exposure to EtBr (1 μg/ml) and (iii) after 60 minutes of exposure to EtBr (1 μg/ml) and CPZ (20 μg/ml) at 37°C without glucose. Data in graphics A1 and A2 correspond to the same assay and were separated for the sake of figure clarity.