Figure 1

Standard assembly of BglBrick parts. Unique BglII (in red) and BamHI (in blue) restriction sites flank BglBrick basic parts on their 5' and 3' ends, respectively. EcoRI and XhoI restriction sites employed in various protocols for part assembly are also shown. Cleavage of each DNA with the appropriate enzyme (color-coded arrowheads) generates compatible cohesive ends. These can be connected head-to-tail by ligation (black arrow) to generate composite parts separated by a 6 nucleotide scar sequence (ggatct). When translated in frame, the scar sequence between parts encodes glycine-serine, a peptide linker innocuous for most protein fusion applications.