Figure 2

Assembly of lacZ expression devices. (A) Standard assembly of BglBrick basic parts were used to generate a series of constitutive lacZ expression devices. Each composite part consisted of a different ribosome binding site part located between promoter and lacZ coding sequence parts. (B) Sequence alignment of RBS variants. Asterisks indicate sequence identity. (C) The β-galactosidase activity of each device (labeled for RBS parts J61140-J61146), along with a negative control lacking a device (DH10B cells alone), is shown. For each, the average of 5 individual replicates is shown. A wide range of activities were observed, indicating the ability to tune protein expression levels using BglBrick RBS part libraries.