Figure 4
From: Insulation of a synthetic hydrogen metabolism circuit in bacteria
Insulation of the hydrogenase pathway through ferredoxin binding surface mutagenesis A.) Homology model of C. reinhardtii hydrogenase with mutated residues highlighted in cyan and spinach ferredoxin X-ray structure (1A70 [54]) with negatively charged residues highlighted in yellow. Iron-sulfur clusters and the hydrogenase catalytic cluster are highlighted in orange. B.) Relative in vitro and in vivo hydrogen production for wild type and mutated C. reinhardtii hydrogenase. Mutants D126K and E5K, which enhance charge-complementarity at the putative interaction surface, show a specific enhancement of in vivo activity relative to activity changes seen in the ferredoxin-independent in vitro assay.