Figure 1

Comparison of constitutive LuxR variants. In these experiments, LuxR was expressed from a tetracycline-inducible promoter, P _LtetO-1 , in strain GN101, which harbors a chromosomal copy of tetR. Activity was determined by the ability of these different variants to induce expression from the P _luxI promoter, using GFP as the readout, in the absence of any autoinducer. Dark bars denote the uninduced case and light bars the induced case (200 ng/mL aTc). Error bars denote 95% confidence intervals.