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Table 1 Experimental design to test the effect of the positive feedback.

From: A positive feedback-based gene circuit to increase the production of a membrane protein

Circuits tested Key Remarks
pKB1+pGN3 PF+CP Treatment
pKB2+pGN3 NPF + CP Control to test the effect of positive feedback
pKB2 NPF Control to test the leakiness of the p lux promoter
pKB1 PF Control to test the combined effect of the leakiness of the promoter and the positive feedback
N/A WT Control to test wild type expression levels