Table 1 Experimental design to test the effect of the positive feedback.
From: A positive feedback-based gene circuit to increase the production of a membrane protein
Circuits tested | Key | Remarks |
|---|---|---|
pKB1+pGN3 | PF+CP | Treatment |
pKB2+pGN3 | NPF + CP | Control to test the effect of positive feedback |
pKB2 | NPF | Control to test the leakiness of the p lux promoter |
pKB1 | PF | Control to test the combined effect of the leakiness of the promoter and the positive feedback |
N/A | WT | Control to test wild type expression levels |