Figure 3
From: Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70
Cell-free protein synthesis of eGFP variants as a function of DNA regulatory parts. Expression kinetics of four eGFP variants, 1 nM plasmid final concentration (3-PGA buffer, conditions: 5 mM magnesium glutamate, 50 mM potassium glutamate, 1.5 mM of each amino acid, 2% PEG 8000). Concentrations of magnesium glutamate and potassium glutamate reported here are the concentrations added to the cell-free reaction. The crude extract, dialyzed against the S30 buffer B, brings an additional 4.5 mM magnesium glutamate and 20 mM potassium glutamate to the cell-free reaction.