Figure 1
From: Rapid optimization of gene dosage in E. coli using DIAL strains
qPCR estimation of plasmid copy number. JTK160 (repA) and JTK164 (pir) variants were transformed with pBjk2992-jtk2541 and pBjk2993-jtk2541, respectively. JTK160J and JTK164J were also transformed with pBjo1601A-jtk2541 (p15a origin) and pBjk3057-jtk2541 (pUC origin). Two biological replicates of each sample were prepared and analyzed using qPCR. Error bars represent standard deviation.