Figure 2
From: Rapid optimization of gene dosage in E. coli using DIAL strains
Flow cytometric analysis of cell-to-cell variation. JTK160 strains expressing repA (A) or JTK164 cells expressing pir (B) bearing GFP expressing plasmids (pBjk2741-jtk3026 or pBjk2807-jtk3026, respectively) were analyzed by flow cytometry. MC1061 alone or bearing p15a (pBjo1601A-jtk3026) or pUC (pBca9145-jtk3026) plasmids expressing GFP were also analyzed. Data is presented as forward scatter versus GFP fluorescence, with the mean and coefficient of variance listed below.