Table 2 Comparison of Alternative Assembly Methods
From: Amplified insert assembly: an optimized approach to standard assembly of BioBrickTM genetic circuits
Standard Assembly | Three Antibiotic Assembly | Amplified Insert Assembly | |
|---|---|---|---|
Plasmid Extraction | X | X | X |
Restriction Digest | X | X | X |
Phosphatase Treatment | X | X | |
DpnI Digest | X | ||
Gel Electrophoresis | X | ||
PCR | X | X | |
Ligation | X | X | X |
Purification | After Gel | Before Transformation | Before Digestion |
Accuracy | Depends on size: Gel extraction difficult for small inserts | 89% | 99% |