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Figure 6 | Journal of Biological Engineering

Figure 6

From: A framework and model system to investigate linear system behavior in Escherichia coli

Figure 6

Testing for the presence of superposition in the model system. For each of the indicated replicons, plasmid copy number (A) and nptII transcript level per plasmid copy (B) were determined for empty backbone and backbone harboring cat or/and gfp devices. Values for each replicon were normalized to that of the empty backbone construct, which was assigned a mean value of one. The difference in value between empty and device harboring backbone was determined statistically for each replicon (P < 0.05, t-test). Asterisks indicate significant difference. C. For each of the indicated replicons, cat transcript level per plasmid copy was determined for backbone containing either cat device alone or both cat and gfp devices. Values for each replicon were normalized to that of the backbone construct with only cat device present, which was assigned a mean value of one. The difference in value between backbone containing either cat device alone or both cat and gfp devices was determined statistically for each replicon (P < 0.05, t-test). Asterisks indicate significant difference. D. For each of the indicated replicons, gfp transcript level per plasmid copy was determined for backbone containing either gfp device alone or both cat and gfp devices. Values for each replicon were normalized to that of the backbone construct with only gfp device present, which was assigned a mean value of one. The difference in value between backbone containing either gfp device alone or both cat and gfp devices was determined statistically for each replicon (P < 0.05, t-test). No significant differences were observed. For all panels, the mean value as determined from two independent experiments (constructs were tested in duplicate during each independent experiment) has been reported. Error bars denote standard error.

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