Table 2 Primers used in RT-PCR reactions
Primer | Sequence 5′ to 3 | Target of primer pair | PCR product, bp |
|---|---|---|---|
ASnifDF1 | tcgtattcggtggtgacaaa | nifD | 204 |
ASnifDR1 | gagacacaccacggaaacct | ||
AShoxHF1 | gaatccgtctgcgtcaattt | hoxH | 284 |
AShoxHR1 | gcaaatgtccgtcgtaggtt | ||
23F | gctaagcgatgtaccgaagc | 23S rDNA | 200 |
23R | taacccagagtggacgaacc | ||
PsaAF1 | ctgttgaaaggtgtattgtt | psaA | 489 |
PsaAR1 | aggagctaccttcagtttat | ||
PsbAF1 | gcacattcaactttatgatt | psbA | 390 |
PsbAR1 | ccaaaattgagttattgaag | ||
FdxHF1 | atggctagctaccaagttag | fdxH | 299 |
FdxHR1 | ttaagcaaggtacggttctt | ||
CoxAF1 | gcgagattacttcagtttta | coxA | 426 |
CoxAR1 | atccaaataccttctcctac | ||
NtcAF1 | cgagtctactttcttttgaa | ntcA | 354 |
NtcAR1 | aaaatcacgacagagaatta | ||
HetRF | ggatgaccggacatttgcac | hetR | 321 |
HetRR | ccataagcgatcgcaagagg |