Mathematical model of the protein production process. (A) Time course simulation results for total GFP production for the no-feedback and three feedback systems over 12 hours. Points represent experimentally measured values. (B) Predicted and experimentally measured soluble GFP fractions after 8 hours for feedback mechanisms with different strength (color map as in 6A). Points represent simulation results. Experimental data are averages over four replicates, where bars +/- standard error of the mean.