Figure 4From: Directed evolution of bright mutants of an oxygen-independent flavin-binding fluorescent protein from Pseudomonas putidaFlavin-binding cavity in FbFP. Amino acids upto a distance of 0.3 nm from FMN are shown. Also shown are A52 and F37 which are at a distance of 0.4 nm from FMN. Amino acids are colored according to their property (blue – aromatic; red - negatively charged; violet - positively charged; green – hydrophobic; deep green - polar). The W94 target lies outside the vicinity of the chromophore and does not appear in the structure.Back to article page