Figure 3

Cellular inflammation in A549 cells following exposure of different concentrations of MWCNTs under static and dynamic cell growth conditions. IL-8 was measured from the media supernatant of A549 cell culture following exposure of MWCNTs at different concentrations (0, 5, 10, and 20 μg/ml) under both cell growth conditions. Each data point from IL-8 measurement (pg/ml) was normalized by total protein concentration (μg/ml) at each time point. Time 0 h refers to the starting point of cyclic equibiaxial deformation. #Significantly different from the other cell growth condition at the same MWCNT concentration and exposure duration (p<0.05). *Significantly different from the control for static cell growth condition (static condition at 0 μg/ml of MWCNT) under the same exposure duration (p<0.05). +Significantly different from the control for dynamic cell growth condition (dynamic condition at 0 μg/ml of MWCNT) under the same exposure duration (p<0.05).