Figure 1

Diagram of plasmids pMU102 and pMU770. Yellow arrows indicate coding sequences, green arrows indicate promoters, red arrows indicate terminators, and grey arrows indicate replication origins. The E. coli origin of replication is derived from plasmid pUC19. The C. thermocellum origin of replication, repB replication gene, and cat antibiotic resistance gene are derived from plasmid pNW33N. The gapDH promoter is derived from C. thermocellum genomic DNA. The rrnB and T7 terminators were synthesized based on publicly available sequence data.