Figure 3

Testing the mf -Lon protease. (a) A three plasmid system was used to test CI degradation by mf-Lon. The circuit has a positive feedback loop consisting of P _RM expressing its activator, CI. Another plasmid expressed mf-Lon when induced with IPTG. Three different ssrA tag conditions were used for the activator (ec-ssrA, mf-ssrA, no tag). When mf-Lon is expressed a properly tagged CI should degrade, thus turning off the positive feedback loop. (b) The PRM-GFP reporter was used to measure the presence of CI. (c) ThePLtetO-1-GFP reporter was used to test the presence of TetR. All data points were measured using three biological replicates. Error bars indicate data range (highest and lowest value).