Figure 3
From: Wide-dynamic-range promoters engineered for cyanobacteria
The L03 promoter strength measured in Synechocystis sp. strain ATCC27184 (i.e. glucose-tolerant Synechocystis sp. strain PCC 6803) cells. Cells were sampled on 24, 48, and 72 hours after induced with 0 (black bar), 102 (blue bar), 103 (green bar), and 104 (magenta bar) ng/mL aTc and grown under different light conditions: light-activated darkness (upper panel), red light (middle panel), and white light (lower panel). The Synechocystis cells were grown in kanamycin-added BG11 medium supplemented with 0 mM (left panel) or 5 mM (right panel) glucose. The EYFP emission per cell has been subtracted with auto-fluorescence of a cell containing pPMQAK1 plasmid only. a.u., arbitrary unit. The detail induction data can be found in Additional file 1: Table S2.