Table 1 Plasmids constructed in this study
From: Targeting a heterologous protein to multiple plant organelles via rationally designed 5′ mRNA tags
pORE-GFP | pORE binary vector expressing untagged soluble modified GFP (smGFP) controlled by the pENTCUP2 promoter [29]. |
pORE-PIMT2-GFP | pORE vector expressing A. thaliana codon-optimized GFP, with an N-terminal chloroplast targeting peptide of protein-L-isoaspartate methyltransferase (PIMT2) [15, 16]. |
pORE-TTL-GFP | pORE vector expressing A. thaliana codon-optimized GFP, with an N-terminal start codon and peroxisome-targeting peptide of transthyretin-like S-allantoin synthase (TTL) [20, 21] |
pORE-TriTag-1-GFP | pORE vector expressing TriTag-1-fused GFP controlled by the pENTCUP2 promoter. |
pORE-TriTag-2-GFP | pORE vector expressing TriTag-2-fused GFP controlled by the pENTCUP2 promoter. |
pORE-TriTag-3-GFP | pORE vector expressing TriTag-3-fused GFP controlled by the pENTCUP2 promoter. |