Table 1 BioBrick parts
From: Design and analysis of a tunable synchronized oscillator
Component | Function | Transcriptional control | Source | ||
|---|---|---|---|---|---|
LuxR | C0062 | AHL-dependent TF | Constitutive | J23101 | Vibrio fischeri |
LuxI-LVA | C0061 | AHL-synthase | Induced by AHL-LuxR Repressed by LacI | I751502 | Vibrio fischeri |
AiiA-LVA | C0060 | AHL-degradase | Induced by AHL-LuxR, Repressed by TetR | K176000 | Bacillus thuringiensis |
GFP-LVA | J04031 | Reporter molecule | Induced by AHL-LuxR | R0062 | Aequeora victoria |
TetR | C0040 | Repressor of aiiA | araC/pBAD | I0500 | Escherichia coli |
LacI | C0012 | Repressor of luxI | araC/pBAD | I0500 | Escherichia coli |
mCherry | J06504 | Constitutive reporter molecule | araC/pBAD | I0500 | Discosoma sp. |
Backbone | pSB1A2 | Cloning & expression vector | N/A | N/A | Escherichia coli |