Figure 6

Isolation of a- or α-type of cells generated via spontaneous chromosomal aberration. (A) Direct images of colony formation in isolation of a-type of cells. Cell suspensions containing approximately 1 × 10⁷ yeast cells (1 mL of suspension at OD₆₀₀ 1.0) were spread on selective solid medium (SD – Ura plate). (B) Fluorescent reporter assay to quantify expression of the GFP reporter gene in yeast transformants possessing plasmid pH2G-Pa1 (P _STE2 -GFP). Ctrl indicates the parental MAT a/α transformant (negative control). (C) Direct images of colony formation in isolation of α-type of cells. Cell suspensions containing approximately 1 × 10⁶ yeast cells (1 mL of suspension at OD₆₀₀ 0.1) were spread on selective solid medium. (D) Fluorescent reporter assay to quantify expression of the GFP reporter gene in yeast transformants possessing plasmid pL3G-2α (P _STE3 -GFP). Ctrl indicates the parental MAT a/α transformant (negative control). (E) Mating assays to investigate the mating abilities of yeast cells. Each spot corresponds to 10 μL of suspension at the indicated OD₆₀₀.