The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis

Table 1 Overview of the Bacillus BioBrick Box including a summary of the features

BioBrick	Description and comments^a	Reference of source	BGSC #	Registry #
Vectors
pBS1C	Empty vector, integration at amyE, amp^r, cm^r	pDG1662-derivative [23]	ECE257	BBa_K823023
pBS2E	Empty vector, integration at lacA, amp^r, mls^r	pAX01-derivative [24]	ECE258	BBa_K823027
pBS4S	Empty vector, integration at thrC, amp^r, spec^r	pDG1731-derivative [23]	ECE259	BBa_K823022
pBS1ClacZ	lacZ-reporter vector, integration at amyE, amp^r, cm^r	pAC6-derivative [25]	ECE260	BBa_K823021
pBS3Clux	lux-reporter vector, integration at sacA, amp^r, cm^r	pAH328-derivative [26]	ECE261	BBa_K823025
Promoters
P_veg	Very strong constitutive promoter	[27]	ECE262	BBa_K823003
P_liaG	Constitutive promoter	[28]	ECE263	BBa_K823000
P_lepA	Strong constitutive promoter	[29]	ECE264	BBa_K823002
J23101	Very weak constitutive promoter	BBa_J23101	ECE266	BBa_K823005
P_liaI	Bacitracin-inducible promoter	[30, 31]	ECE267	BBa_K823001
P_xylA	Xylose-inducible promoter	[32]	ECE268	BBa_K823015
Epitope-tags
His₁₀	10xHis-tag	[33]	ECE269	BBa_K823037
FLAG	FLAG-tag	[34]	ECE270	BBa_K823034
StrepII	Streptactin-tag	[35, 36]	ECE271	BBa_K823038
HA	HA-tag	[34, 37]	ECE272	BBa_K823035
cMyc	cMyc-tag	[34, 38]	ECE273	BBa_K823036

^aamp^r, ampicillin resistance; cm^r, chloramphenicol resistance; kan^r, kanamycin resistance; spc^r, spectinomycin resistance; mls^r, erythromycin-induced resistance to m acrolide, l incosamide and s treptogramin B-antibiotics (MLS).

ISSN: 1754-1611