The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis

Table 2 Vector sources for the Bacillus BioBrick Box

Vector	Description^a	Used for	Ref.
pAC6	Vector for transcriptional promoter fusions to lacZ; integrates at amyE; cm^r, amp^r	pBS1ClacZ	[25]
pAH328	Vector for transcriptional promoter fusions to luxABCDE (luciferase); integrates at sacA; cm^r, amp^r	pBS3Clux	[26]
pDG1662	Empty vector, integrates at amyE, cm^r, spc^r, amp^r	pBS1C	[23]
pDG1731	Empty vector; integrates at thrC, spc^r, mls^r, amp^r	pBS4S	[23]
pAX01	Vector for xylose-dependent gene expression; integrates at lacA, mls^r, amp^r	pBS2E	[24]
pXT	Vector for xylose-inducible gene expression; integrates in thrC; spc^r, amp^r	P_xylA	[39]
pSB1C3	Replicative E. coli vector, MCS features rfp-cassette; cm^r	MCS	[40]
pGFPamy	Vector for transcriptional promoter fusions to gfpmut1; integrates at amyE; cm^r, amp^r	gfpmut1	[41]

^acm^r, chloramphenicol resistance; kan^r, kanamycin resistance; spc^r, spectinomycin resistance; mls^r, erythromycin-induced resistance to m acrolid-, l incosamid- and s treptogramin B-antibiotics (MLS); MCS, multiple cloning site.

ISSN: 1754-1611