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Table 2 Vector sources for the Bacillus BioBrick Box

From: The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis

Vector Descriptiona Used for Ref.
pAC6 Vector for transcriptional promoter fusions to lacZ; integrates at amyE; cmr, ampr pBS1ClacZ [25]
pAH328 Vector for transcriptional promoter fusions to luxABCDE (luciferase); integrates at sacA; cmr, ampr pBS3Clux [26]
pDG1662 Empty vector, integrates at amyE, cmr, spcr, ampr pBS1C [23]
pDG1731 Empty vector; integrates at thrC, spcr, mlsr, ampr pBS4S [23]
pAX01 Vector for xylose-dependent gene expression; integrates at lacA, mlsr, ampr pBS2E [24]
pXT Vector for xylose-inducible gene expression; integrates in thrC; spcr, ampr P xylA [39]
pSB1C3 Replicative E. coli vector, MCS features rfp-cassette; cmr MCS [40]
pGFPamy Vector for transcriptional promoter fusions to gfpmut1; integrates at amyE; cmr, ampr gfpmut1 [41]
  1. acmr, chloramphenicol resistance; kanr, kanamycin resistance; spcr, spectinomycin resistance; mlsr, erythromycin-induced resistance to m acrolid-, l incosamid- and s treptogramin B-antibiotics (MLS); MCS, multiple cloning site.